WebDec 23, 2024 · RAGE is a transmembrane receptor whose activation plays an important role in mediating pro-inflammatory responses. 25 HMGB1 interacts with RAGE to increase the permeability and rupture of the lysosome membrane, activate and release cathepsin B, 8 which directly activates NLRP3 through the leucine-rich repeat (LRR) domain of NLRP3, … WebApr 3, 2024 · The major source of circulating HMGB1 in sepsis is hepatocytes. However, the mechanism of HMGB1 release of hepatocytes during sepsis is not very clear. We have previously shown that bacterial endotoxin [lipopolysaccharide (LPS)] sensing pathways, including Toll-like receptor (TLR)4 and caspase-11, regulate hepatocyte HMGB1 release …
HMGB1/RAGE pro-inflammatory axis promotes vascular …
WebHigh mobility group box 1 (HMGB1) is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death; it is the prototypic … WebCo-IP (co-immunoprecipitation) analysis further confirmed that the interaction between HMGB1 and RAGE could be blocked with FPS-ZM1 (RAGE inhibitor) (Fig. 3b). … rsi wheels
RAGE signaling pathways: Ligands (such as AGEs, S100, HMGB1, …
Next, we investigated how the HMGB1 was loaded into the EVs and released into the extracellular space. First, we observed that the RAGE was co-localized with the endosome system (EEA1, Rab5, and Rab7). When LPS-induced HMGB1 translocated from the nucleus to the cytoplasm, cytoplasmic HMGB1 co … See more We isolated serum EVs (Fig. 1a) from sepsis patients (patient information is shown in Table 1). The common markers of the EVs (CD63, … See more Next, we chose AML-12 (hepatocyte cell line) as the recipient cells of the macrophage-released EVs. With the transwell system, we … See more From the clinical data, we found that the level of HMGB1+ serum-EVs was positively correlated with the level of liver injury in the patient (Fig. 2a–b) and the inflammation index (C-reactive protein (CRP) and … See more To find the function of LPS-EVs, we focused on the biological effects of the LPS-EVs on hepatocytes because we already found that LPS-induced macrophage EVs cause liver damage. We found that LPS-EVs … See more WebApr 1, 2003 · Increased smooth muscle migration following HMGB1 binding to RAGE is mediated through a pertussis toxin–sensitive pathway and blocked by ... (RAGE) is a cellular binding site for amphoterin: mediation of neurite outgrowth and co-expression of rage and amphoterin in the developing nervous system. J Biol Chem. 270. 1995. 25752. 25761. … WebBy real-time-PCR, mRNA-expression levels of HMGB1 and RAGE were upregulated in muscle biopsies of patients with IBM and PM, but not in muscular dystrophy or non-myopathic controls. By immunohistochemistry, both molecules displayed the highest signal in IBM, where they distinctly co-localized to intra-fiber accumulations of β-amyloid and ... rsi wilmington vt