2024 Fl83b cells

Fl83b cells

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WebApr 1, 1973 · FL83B is a normal cell line of hepatocytes from a C57BL/6J mouse of 15–17 days of gestation (Breslow et al., 1973). FL83B required F-12 K medium with 10% FBS. … WebJan 11, 2024 · d FL83B cells were cultured with different concentrations (as indicated) of PC (34:2) for 16 h, and the resulting effects on AhR expression were determined by western blots. Ratio to β-actin ...

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WebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... builder hourly rate

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WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … WebOriginal magnification: 100×. (C) FL83B cells were transfected with HA-Daxx and then treated with or without TGF-β for 24 h. Western blotting analysis of Daxx, E-cadherin and Vimentin expression. Relative expression was normalized to β-actin expression as a reference. The graph shows quantitative densitometry from Western blotting. WebS-Allyl cysteine attenuates free fatty acid-induced lipogenesis in human HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway ☆ Author links open overlay panel Yong Pil Hwang a b 1 , Hyung Gyun Kim a 1 , Jae Ho Choi a , Minh Truong Do a , Young Chul Chung c , Tae Cheon Jeong d , Hye Gwang Jeong a crossword dictionary free online tool

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FAM83B is involved in thyroid cancer cell differentiation and …

WebFL83B cells were obtained from the Taiwan Bioresource Collection and Research Center (BCRC number: 60325) and cultured in F12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO 2. To make the stock medium containing 4 mM OLA, sodium oleate was conjugated … WebThe FL83B cells were treated with insulin for 5 or 10 min. GRK2 was labeled with anti-GRK2 antibody and Cy3-conjugated secondary antibody and the cells were observed under the microscope. Insulin treatment increased the translocation of GRK2 from cytosol to the plasma membrane ( Fig. 1A ). crossword diminish or shrink graduallyWebJun 15, 2015 · Abstract We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking … builder hub whirlpool

"WebAug 4, 2024 · Cytotoxicity of PG/Dox synergism against normal cells. To access putative toxicity from PG/Dox synergism in liver, kidney, and heart, FL83B (murine normal hepatocyte), HK-2 (human kidney proximal tubule epithelial cell), and h9c2 (murine normal cardio-myoblast) were sequentially treated with PG and Dox for 12 h, respectively. " - Fl83b cells

Fl83b cells

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WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its … WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot …

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WebJul 13, 2024 · Characteristics. tissue/cell type: Liver. condition: Healthy. treatment: none. Treatment protocol. FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non … WebFeb 6, 2013 · FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, …

WebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ... Web2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modiﬁed from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor

WebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … WebMay 8, 2024 · The effects of a triterpene purified from bitter melon was investigated against TNF-α-induced inflammation via AMP-activated protein kinase in FL83B cells. This compound suppressed the TNF-α-induced expression of inflammatory markers, including inducible NO synthase, p65 subunit of NF-κB, TNF-α, and IL-1β (Cheng et al., 2012).

WebIn another experiment, FL83B cells were challenged with oleic acid to induce lipid accumulation and treated with various concentrations of fisetin. Results: NAFLD mice treated with fisetin had decreased body weight and epididymal adipose tissue weight compared to NAFLD mice. Fisetin treatment also reduced liver lipid droplet and …

crossword dinWebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ... builder houseWebHowever, whether reversibility exists in mesenchymal stromal cell (MSC)-derived hepatocytes (dHeps) remains elusive. In this study, we find that hepatogenic differentiation (HD) of MSCs is a reversible process and is modulated by DNA methyltransferases (DNMTs). DNMTs are regulated by transforming growth factor β1 (TGFβ1), which in turn ... builder hourly rate ukWebJul 1, 2014 · FL83B cell morphology before and after DEHP exposure at different concentrations are shown in Fig. 1. With the increase of DEHP concentration, cells tend to shrink. Afterward, the cell lost its ability to adhere on the plate of the dish; thus, it was found floating in the media. Beside shrinkage, cells also showed decrease in cell density. crossword dilly dallyWebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N … builder hunter bright brass ceiling fanWebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL … builder house near meWebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the … crossword dingy